Analytical evaluation of eight-cell cow embryos structure, outwards water and inwards cryo-protectants to ensure successful cryopreservation select="/dri:document/dri:meta/dri:pageMeta/dri:metadata[@element='title']/node()"/>

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dc.contributor.author Mausse, Francisco
dc.contributor.author Bezugly, N.
dc.date.accessioned 2014-06-30T11:23:56Z
dc.date.available 2014-06-30T11:23:56Z
dc.date.issued 2013
dc.identifier.citation Mausse, F., & Bezugly, N. (2013). Analytical evaluation of eight-cell cow embryos structure, outwards water and inwards cryo-protectants to ensure successful cryopreservation. International Science and Technology Journal of Namibia, 2(1), 70-77. en_US
dc.identifier.issn 20267673
dc.identifier.uri http://hdl.handle.net/11070/1123
dc.description.abstract Modern reproductive techniques use cow embryos at different stages of development. Eight-cell embryos feature a layer comprising seven cells (blastomeres), surrounding the inner cell, making a total of eight. This article analyses outwards water paths and inwards cryo-protectants, within the eight-cell cow embryos and effectiveness of their cryopreservation. Reproductive cycles and superovulation in cows were triggered through reproductive hormones injection. After slaughter, oocytes were harvested and eight-cell embryos were produced in vitro fertilization and through embryo culture. The study included morphological observations to visualize embryo structure, distribution of blastomeres, cell-layers and analytical evaluation as well, to assess distribution of penetrating cryoprotectants within the inner-cell mass. Findings based on the eight-cell embryo structure suggest that the quantity of outwards water and inwards cryo-protectants is symmetrically distributed. Although, the characteristic of water and cryo-protectants movement is expected to be much better in eight-cell embryos, cryo-protectants properties and the modality of cryo-protectants should differ from procedures applied in other class of embryos. Survival rate of cryopreserved eight cell embryos was lower (6.3%) as compared to the results for 7–8 days embryos. Obviously, a successful cryopreservation of eight-cell cow embryos can only be achieved through a combination of both technical and biological parameters. en_US
dc.language.iso en en_US
dc.publisher University of Namibia en_US
dc.subject Water and cryo-protectants en_US
dc.subject Eight-cell embryos and penetrability en_US
dc.title Analytical evaluation of eight-cell cow embryos structure, outwards water and inwards cryo-protectants to ensure successful cryopreservation en_US
dc.type Article en_US


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